The strains selected in the previous paper were identified according to the manual of Aspergilli by Thom and Raper, and the preparation of the crude enzymes with the strains were investigated. The fermented fodders were made with wheat bran and chaffs containing 20% wheat bran by the strains, and the feeding experiments concerned was conducted.
The results obtained are as follows:
1. The selected strains were identified as below;
MC-9 was a similar strain of Aspergillus niger van Tieghem,
MC-10 was a similar strain of Aspergillus flavus Link,
MC-53 was a similar strain of Aspergillus penicilloides Spegazzini and MC-61 was a similar strain of Aspergillus niger rout Schiemanni n. comb.
2. Percentages of crude enzymes obtained from the dried matters of the wheat bran cultures were MC-9: 2.9%, MC-10: 3.9%, MC-53: 6.4% and MC-61:2.6%. And the cellulase activity of MC-9 was the most active among the crude enzymes as the same as the filtrates of wheat brat cultures.
3. As the results of the feeding experiments, changes of body weights showed no trends to be significant as compared with the control group, and the groups of MC-9 and MC-31 showed the increasing significance with P$lt;0.05 in egg-laying rate.
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